Cationic amino acid transport into cultured animal cells. I. Influx into cultured human fibroblasts.
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چکیده
منابع مشابه
COMMENTARY Introducing proteins into cultured animal cells
Improved methods of protein purification and antibody production, together with a burgeoning array of techniques for single-cell analysis, have led increasing numbers of cell biologists to try microinjecting functional proteins, or antibodies raised against them, into living cells. This has highlighted the shortcomings of available techniques, and encouraged attempts to develop new methodologie...
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Amino acid transport into McCoy cells infected with strains representative of the two major biovars of Chlamydia trachomatis has been studied to determine if uptake is increased during infection. Preliminary work suggested that the transport systems L, A/ASC (for neutral amino acid transport), N (for transport of Asn, Gln, and His) and y+ (for cationic amino acids) were present in McCoy cells. ...
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The transport of neutral amino acids and their interactions for uptake have been studied in skin-derived-diploid human fibroblasts cultured at confluency. Properly timed preincubations in a medium defined in composition and requirements have been adopted to control size of internal amino acid pools and regulatory interferences affecting amino acid transport. L-Proline, L-alanine, and L-leucine ...
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The regulation of the transport of neutral amino acids across the cell membrane by adaptive mechanisms has been studied in cultured human fibroblasts. Among the three transport systems (A, ASC, and L) individually discriminated, only the Na+-dependent System A was subject to adaptive regulation, showing enhancement of its activity when the cells were incubated under conditions of amino acid sho...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34742-2